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KMID : 0861020120270020037
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Korea Journal of Herbology
2012 Volume.27 No. 2 p.37 ~ p.42
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Study of Antioxidant Effects of Platycladi Semen on Male Reproductive cells
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Kim Ju-Sung
Hong Min-Jung
Kim Do-Rim
Park Eun-Hwa
Chang Mun-Seog
Park Seong-Kyu
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Abstract
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Objectives: The purpose of this study is to examine the antioxidant effects on male mouse reproductive cells of
the extract of Platycladi Semen.
Methods: The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, cell viability
by a modified MTT assay, the effects on H2O2-induced cytotoxicity by MTT assay, lipid peroxidation by
malondialdehyde (MDA) formation and super oxide dismutase (SOD), respectively.
Results: The results showed that the extract scavenged DPPH radical in a dose-dependent manner by up to
74.87%. The cell viability of the extract was within 72～96% on Leydig cells and GC-2 cells at concentrations
of 1, 5, 10, 50 and 100 ug/ml. The hydrogen peroxide-induced cytotoxicity of Leydig cells was protected to
72.09% by the extract at concentration of 100 μg/ml. The hydrogen peroxide-induced lipid peroxidation of
MDA formation was decreased to 1.80 and 1.65 nmoles/mg protein by the extract at concentrations of 50 and
100 μg/ml. The extract at all concentrations, SOD activity was not significantly changed.
Conclusions: In conclusion, the extract of Platycladi Semen has antioxidant effects on Leydig cells and protect
male reproductive system against oxidative stress.
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KEYWORD
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Platycladi Semen, diphenyl-picryl-hydrazyl (DPPH), MTT assay, hydrogen peroxide (), lipid peroxidation (LPO), Leydig cells
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